Jade Express™ Mix-ROX

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Quantitative real-time PCR (qPCR)
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Purpose 2X qPCR Master Mix
Characteristics Jade ExpressTM Mix-ROX provides a convenient, reliable and robust set-up for performing for extremely rapid quantitative PCR (real-time analysis) of DNA samples in a premixed and optimized format. Designed specifically for the aforementioned niche of application, the components of Jade ExpressTM 2X Master Mix promise top notch performance with respect to sensitivity, signal-to-noise ratio and elimination of primer dimers. The use of TaqFast DNA Polymerase in Jade ExpressTM 2X QPCR Master Mix-ROX shortens the reaction time to about 1/5 of standard qPCR time. Available with the option of ROX or fluorescein as the internal passive reference dye, Jade ExpressTM 2X QPCR Master Mix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. In light of the fact that the QPCR instruments can vary from user to user, we offer the Jade ExpressTM 2X Master Mix in a range of formulations, each of which has been carefully optimized to confer the best performance according to the make and model of a QPCR machine. Please use QPCR Mix that will be most compatible with your choice of a particular instrument/model. Jade ExpressTM 2X Master Mix-ROX (Cat# M1112-500) is compatible with ABI® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series QPCR instruments
Components Jade ExpressTM Mix-ROX 2X QPCR mixture is a 2X concentrated mix of TaqFast DNA Polymerase, dNTPs, MgCl2, fluorescent dye (detection), reference dye and proprietary buffer components.
Application Notes - Gene-expression analysis
- Gene cloning
- Microarray validation
- High throughput applications
- Virus detection and quantification

- Extremely rapid activation - only 30 seconds!
- Ultra-fast qPCR thermal cycling - 10 second annealing/elongation time
- Streamlined protocol with a simple reaction set-up
- Ultra-fast quantification of a variety of gene targets
- Reduces pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments

Protocol For ultrafast PCR, conferring a significant reduction to the overall QPCR quantification and detection time thus streamlining the experiment through cost and labor saving.
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the Jade ExpressTM 2X Master Mix-ROX should be stored at -20°C and protected from light. After each experiment, the leftover mix (completely thawed and thoroughly homogenized) can be stored at 4°C if it is to be used within the next 3 months. Avoid repeated freezethaw cycles to retain maximum performance. The Jade ExpressTM 2X Master Mix-ROX is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months