Jade Smart™ Mix-iCycler

Details for Product No. ABIN5684212, Supplier: Log in to see
Application
Quantitative real-time PCR (qPCR)
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Purpose 2X qPCR Master Mix
Characteristics Jade SmartTM Mix-iCycler provides a convenient, reliable and robust set-up for amplifying long DNA targets in a premixed and optimized format. Designed specifically for the for the quantitative amplification of DNA fragments ranging in length from 300bp to 1.3kb. This 2X QPCR Master Mix has been specially optimized to amplify DNA targets with varying lengths and GC contents. It is also able to overcome DNA fragments that lead to problematic secondary structures. Available with the option of ROX or fluorescein as the internal passive reference dye, Jade SmartTM Mix-iCycler enables reliable amplification and offers unmatched performance in sensitivity and signal-to-noise ratio. Due to variations in QPCR instrument performance, we offer different Jade SmartTM 2X QPCR Master Mix formulations optimized for different machines. Please refer to the appropriate QPCR Master Mix for your particular instrument model. Jade SmartTM Mix-iCycler (Cat# M1114-500) is compatible with - BioRad® iCycler®, iQ™5, MyiQ™ QPCR instruments.
Components Jade SmartTM 2X QPCR Master Mix is a 2X mix of dNTPs, Hotstart Taq polymerase, MgCl2, fluorescent detection dye, reference dye, and proprietary buffer components.
Application Notes - Gene-expression analysis
- Next generation DNA sequencing
- Gene knockdown validation
- SNP genotyping assays
- CHiP
- Copy number variation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Comment

- Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments

Protocol To amplify DNA targets with varying lengths, GC contents and to overcome DNA fragments that lead to problematic secondary structures
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the Jade SmartTM 2X QPCR Mix should be stored at -20°C and protected from light. After each experiment, the leftover mix (completely thawed and thoroughly homogenized) can be stored at 4°C if it is to be used within the next 3 months. Avoid repeated freeze-thaw cycles to retain maximum performance. The Jade SmartTM 2X QPCR Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months