Jade miRNA™ Mix-iCycler

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Application
Quantitative real-time PCR (qPCR)
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Purpose 2X qPCR Master Mix
Characteristics Jade miRNATM Mix-iCycler is designed specifically for quantitative real-time analysis of miRNA obtained from RNA samples. The components of Jade miRNA 2X QPCR Master Mix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our Master Mix significantly reduces non-specific PCR amplification observed with regular Taq polymerase. Instrument specific Jade miRNA QPCR 2X Master Mix is specially formulated to ensure optimal performance, including high sensitivity, reproducibility and primer specificity. The Master Mix is also designed to eliminate primer dimer formation and amplification of premature miRNAs. Due to variations in QPCR instrument performance, we offer different Jade miRNATM 2X QPCR Master Mix formulations optimized for different machines. Please use 2X QPCR Master Mix (Cat# M1118-500) that will be most compatible with your choice of a particular instrument/model. Jade miRNATM 2X Master Mix-iCycler is compatible with -BioRad® iCycler®, iQ™5, MyiQ™ QPCR instruments.
Components Jade miRNATM Mix-iCycler, 2X QPCR Master Mix is a 2X mix of dNTPs, Hotstart Taq polymerase, MgCl2, fluorescent detection dye, reference dye, and proprietary buffer components
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Jade miRNA™ Mix-iCycler

Protocol For quantitative real time analysis of miRNA obtained from RNA samples
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the Jade miRNATM 2X Master Mix should be stored at -20°C and protected from light. After each experiment, the remaining mixture can be stored at 4°C if it is to be used within the next 7 days, However, freshly thawed Master Mix is highly recommended for optimal results. Avoid repeated freeze-thaw cycles to retain maximum performance. Jade miRNATM QPCR Master Mix-iCycler is stable for 1 year from the date of shipping when stored and handled properly
Expiry Date 12 months