Jade™ Master Mix

Details for Product No. ABIN5684217, Supplier: Log in to see
Application
Quantitative real-time PCR (qPCR)
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Purpose 2X qPCR Master Mix
Characteristics JadeTM Master Mix provides a convenient, reliable and robust set-up for performing quantitative PCR (real-time analysis) of DNA samples in a premixed and optimized format. Designed specifically for the aforementioned niche of application, the components of JadeTM 2X Master Mix promise top notch performance with respect to sensitivity, signal-to-noise ratio and elimination of primer dimers. Furthermore, our proprietary chemical modification of the DNA polymerase included in this Mix allows for Hot Start PCR, conferring a significant reduction in non-specific PCR amplification that is otherwise of a common occurrence with regular Taq polymerases. In light of the fact that the QPCR instruments can vary from user to user, BioVision offers the JadeTM 2X Master Mix in a range of formulations, each of which has been carefully optimized to confer the best performance according to the make and model of a QPCR machine. Please use the QPCR Mix that will be most compatible with your choice of a particular instrument/model. JadeTM Master Mix (#M1105-500) is compatible with BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™, Roche LightCycler® (2.0, 1.5, 480, 1536, Nano), MJ Research Opticon™, Opticon™2, Chromo® 4, Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) QPCR instruments.
Application Notes - Gene-expression analysis
- Gene knockdown validation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Comment

- Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments

Protocol For hot start PCR, conferring a significant reduction in non-specific PCR amplification
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the JadeTM 2X Master Mix should be stored at -20°C and protected from light. After each experiment, the leftover mix (completely thawed and thoroughly homogenized) can be stored at 4°C if it is to be used within the next 3 months. Avoid repeated freeze-thaw cycles to retain maximum performance. The JadeTM 2X Master Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months