Novo™ Reverse Transcriptase

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Purpose NovoTM Reverse Transcriptase is the ultimate solution to synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content).
Characteristics NovoTM Reverse Transcriptase is a novel (NovoTM) recombinant reverse transcriptase (RTase) that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. The Novo™ Reverse Transcriptase is engineered to perform under high temperatures (50 °C-55 °C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, Novo™ can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, Novo™ RTase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Components NovoTM Reverse Transcriptase (200 U/μl), 5X RT Buffer
Application Notes - Synthesizing cDNA from ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Constructing libraries for serial analysis for gene expression (SAGE)
- Synthesizing cDNA in rapid amplification of cDNA ends (3' & 5' RACE)
- Producing template for use in RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization
Restrictions For Research Use only
Storage -20 °C
Storage Comment -20°C
Expiry Date 12 months