Novo™ Reverse Transcriptase

Details for Product No. ABIN5684224,
Purpose NovoTM Reverse Transcriptase is the ultimate solution to synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content).
Characteristics NovoTM Reverse Transcriptase is a novel (NovoTM) recombinant reverse transcriptase (RTase) that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. The Novo™ Reverse Transcriptase is engineered to perform under high temperatures (50 °C-55 °C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, Novo™ can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, Novo™ RTase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Components NovoTM Reverse Transcriptase (200 U/μl), 5X RT Buffer
Application Notes - Synthesizing cDNA from ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Constructing libraries for serial analysis for gene expression (SAGE)
- Synthesizing cDNA in rapid amplification of cDNA ends (3' & 5' RACE)
- Producing template for use in RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization
Restrictions For Research Use only
Storage -20 °C
Storage Comment -20°C
Expiry Date 12 months