Ready™ PCR Mix

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Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix
Characteristics ReadyTM Master Mix is 2X PCR Master Mix is a ready-to-use mixture of high-quality ReadyTM Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for amplification of DNA. To set up a PCR reaction: add DNA template, primers and water. PCR products that are amplified up to 6 kb in length with ReadyTM Taq DNA Polymerase contain a single base (A) 3' overhang.
Application Notes - Routine PCR amplification of DNA templates up to 6 kb
- Suitable for a wide range of PCR assays
- TA cloning

- Robust PCR performance with great reproducibility
- Saves preparation time by combining ReadyTM Taq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- High sensitivity

Protocol PCR amplification of DNA templates up to 6 kb
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the ReadyTM 2X Master Mix should be stored at -20°C. 2X PCR ReadyTM Taq Master Mix are stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The ReadyTM 2X PCR Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months