ReadyTM PCR Master Mix-Dye provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, BioVision offers a variety of highly sensitive Master Mixes to accommodate a wide range of DNA templates and performance needs. ReadyTM PCR Master Mix is a ready-to-use mixture of high-quality ReadyTM Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for the amplification of DNA. The ReadyTM PCR Master Mix with dye contains an inert blue dye (Bromophenol Blue, with migration equivalent to 500 bp DNA fragment) and a stabilizer which allow direct loading of the final products onto a gel for analysis. To set up a PCR reaction: add DNA template, primers and water. PCR products that are amplified up to 6 kb in length with Taq DNA Polymerase contain a single base (A) 3'overhang.