Ready™ PCR Mix-Dye

Details for Product No. ABIN5684232, Supplier: Log in to see
Application
Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix with dye
Characteristics ReadyTM PCR Master Mix-Dye provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, BioVision offers a variety of highly sensitive Master Mixes to accommodate a wide range of DNA templates and performance needs. ReadyTM PCR Master Mix is a ready-to-use mixture of high-quality ReadyTM Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for the amplification of DNA. The ReadyTM PCR Master Mix with dye contains an inert blue dye (Bromophenol Blue, with migration equivalent to 500 bp DNA fragment) and a stabilizer which allow direct loading of the final products onto a gel for analysis. To set up a PCR reaction: add DNA template, primers and water. PCR products that are amplified up to 6 kb in length with Taq DNA Polymerase contain a single base (A) 3'overhang.
Application Notes - Routine PCR amplification of DNA templates up to 6 kb
- Suitable for a wide range of PCR assays
- TA cloning
Comment

- Robust PCR performance with great reproducibility
- Saves preparation time by combining ReadyTM Taq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- High sensitivity

Protocol PCR amplification of DNA templates up to 6 kb
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the ReadyTM 2X Master Mix-Dye should be stored at -20°C. 2X PCR ReadyTM Master Mix are stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The ReadyTM 2X Master Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months