Rigor™ PCR Mix

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Application
Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix
Characteristics RigorTM PCR Master Mix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. The newly introduced 2X PCR Rigor™ Master Mix is a ready-to-use mixture containing BioVision's Rigor™ Taq DNA polymerase, dNTPs, and reaction buffer with proprietary additives in a 2X concentration. It contains all the necessary reagents for reproducible, efficient and high yield amplification of DNA. The Rigor™ Taq DNA polymerase is a thermostable product in BioVision's PCR series with outstanding fidelity due to its 3' to 5' proofreading activity.
Rigor™ Taq DNA polymerase possesses excellent thermostability which allows it to replicate DNA at 70 °C - 80 °C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90 kDa. Unlike Taq DNA Polymerase, Rigor™ Taq DNA Polymerase exhibits 3' to 5' exonuclease (proofreading) activity, which enables the polymerase to correct errors in nucleotide incorporation. As a results, the error rate of Rigor™ Taq DNA Polymerase is 2.6 x 10-6 per nucleotide per cycle. The enzyme is ideal for PCR applications that demand high fidelity and can amplify templates up to 6 kb in length. The amplified PCR products are blunt-ended.
Application Notes - Whole genome sequencing
- Site-directed mutagenesis
- Blunt-end cloning
- Specific amplification of difficult templates (i.e. GC-Rich)
- Low copy PCR assays
Comment

- Saves preparation time by combining RigorTM Taq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- Outstanding accuracy and efficiency using RigorTM Taq DNA Polymerase
- Error-free PCR products for downstream processes

Protocol For specific amplification of difficult templates (i.e. GC-Rich) and low copy PCR assays
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the RigorTM 2X PCR Mix should be stored at -20°C. RigorTM PCR Master Mix is stable at 4°C for three months or for fifteen freezethaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The RigorTM 2X Master Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months