Rigor™ PCR Mix-Dye

Details for Product No. ABIN5684234,
Polymerase Chain Reaction (PCR)
Purpose 2X PCR MasterMix with dye
Characteristics RigorTM 2X PCR Master Mix (with Dye) provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. The Rigor™ Taq DNA polymerase is a thermostable product in BioVision's PCR series with outstanding fidelity due to its 3' to 5' proofreading activity. The newly introduced 2X PCR Rigor™ Master Mix is a ready-to-use mixture containing BioVision's Rigor™ Taq DNA polymerase, dNTPs, and reaction buffer with proprietary additives in a 2X concentration. It contains all the necessary reagents for reproducible, efficient and high yield amplification of DNA. RigorTM 2X PCR Mix (with Dye) includes an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Rigor™ Taq DNA polymerase possesses excellent thermostability which allows it to replicate DNA at 70 °C - 80 °C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90 kDa. Unlike Taq DNA Polymerase, Rigor™ Taq DNA Polymerase exhibits 3' to 5' exonuclease (proofreading) activity, which enables the polymerase to correct errors in nucleotide incorporation. As a results, the error rate of Rigor™ Taq DNA Polymerase is 2.6 x 10-6 per nucleotide per cycle. The enzyme is ideal for PCR applications that demand high fidelity and can amplify templates up to 6 kb in length. The amplified PCR products are blunt-ended.
Application Notes - Whole genome sequencing
- Site-directed mutagenesis
- Blunt-end cloning
- Specific amplification of difficult templates (i.e. GC-Rich)
- Low copy PCR assays

- Saves preparation time by combining RigorTM Taq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- Outstanding accuracy and efficiency using RigorTM Taq DNA Polymerase
- Error-free PCR products for downstream processes

Protocol For specific amplification of difficult templates (i.e. GC-Rich) and low copy PCR assays
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the RigorTM 2X Master Mix (with Dye) should be stored at -20°C. RigorTM 2X PCR Mix is stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The RigorTM 2X PCR Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months