RigorTM 2X PCR Master Mix (with Dye) provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. The Rigor™ Taq DNA polymerase is a thermostable product in BioVision's PCR series with outstanding fidelity due to its 3' to 5' proofreading activity. The newly introduced 2X PCR Rigor™ Master Mix is a ready-to-use mixture containing BioVision's Rigor™ Taq DNA polymerase, dNTPs, and reaction buffer with proprietary additives in a 2X concentration. It contains all the necessary reagents for reproducible, efficient and high yield amplification of DNA. RigorTM 2X PCR Mix (with Dye) includes an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Rigor™ Taq DNA polymerase possesses excellent thermostability which allows it to replicate DNA at 70 °C - 80 °C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90 kDa. Unlike Taq DNA Polymerase, Rigor™ Taq DNA Polymerase exhibits 3' to 5' exonuclease (proofreading) activity, which enables the polymerase to correct errors in nucleotide incorporation. As a results, the error rate of Rigor™ Taq DNA Polymerase is 2.6 x 10-6 per nucleotide per cycle. The enzyme is ideal for PCR applications that demand high fidelity and can amplify templates up to 6 kb in length. The amplified PCR products are blunt-ended.