Robust ReadyTM 2X PCR Master Mix (with Dye) is a ready-to-use mixture of high-quality Robust ReadyTM Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for amplification of DNA. To set up a PCR reaction: add DNA template, primers and water. PCR products, amplified up to 6 kb in length with Robust ReadyTM Taq DNA Polymerase, contain a mixture of blunt ends and single base (A) 3' overhang. The error rate of this PCR amplification is 7.5 x 10-6 per nucleotide per cycle. The products can be used for direct T/A cloning, but its efficiency is not as high as PCR products amplified with Taq DNA Polymerase alone. Robust ReadyTM Master Mix-Dye includes an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.