Robust Ready™ PCR Mix-Dye

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Polymerase Chain Reaction (PCR)
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Purpose 2X PCR MasterMix with dye
Characteristics Robust ReadyTM 2X PCR Master Mix (with Dye) is a ready-to-use mixture of high-quality Robust ReadyTM Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for amplification of DNA. To set up a PCR reaction: add DNA template, primers and water. PCR products, amplified up to 6 kb in length with Robust ReadyTM Taq DNA Polymerase, contain a mixture of blunt ends and single base (A) 3' overhang. The error rate of this PCR amplification is 7.5 x 10-6 per nucleotide per cycle. The products can be used for direct T/A cloning, but its efficiency is not as high as PCR products amplified with Taq DNA Polymerase alone. Robust ReadyTM Master Mix-Dye includes an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Application Notes - Routine PCR amplification of DNA templates up to 6 kb
- Suitable for a wide range of PCR assays
- TA cloning

- Saves preparation time by combining Robust ReadyTM Taq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture
- Reduces the risk of contamination by decreasing the number of pipetting steps
- Provides consistent reaction performance and results
- Improved sensitivity and fidelity as compared to the conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase

Protocol Contains high quality Taq DNA Polymerase offering improved sensitivity and fidelity
Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Upon arrival, the Robust ReadyTM 2X Master Mix should be stored at -20°C. The 2X PCR Robust ReadyTM Mix are stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C. The Robust ReadyTM 2X Master Mix is stable for 1 year from the date of shipping when stored and handled properly. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months