Taqman Master Mix

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Application
Quantitative real-time PCR (qPCR)
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Purpose 2X qPCR Master Mix
Characteristics Taqman Master Mix is designed specifically for TaqMan probe-based real-time PCR analysis of DNA samples. Taqman 2X QPCR Master Mix is designed for high throughput quantitative PCR using TaqMan® probe-based chemistry. Available with the option of ROX or fluorescein as the internal passive reference dye, TaqProbe 2X QPCR Master Mix offers superb performance in sensitivity and signal-tonoise ratio. The multiplex formulation supports quantitative amplification and detection of up to four targets simultaneously with consistent and reliable results. Due to variations in QPCR instrument performance, we offer different Taqman QPCR Master Mix formulations optimized for different machines. Please use BioVision's QPCR Mix that will be most compatible with your choice of a particular instrument/model. Taqman 2X Master Mix (Cat# 1121-500) is compatible with BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™, Roche LightCycler® (2.0, 1.5, 480, 1536, Nano), MJ Research Opticon™, Opticon™ 2, Chromo® 4, Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) QPCR instruments.
Application Notes - Gene-expression analysis
- Gene knockdown validation
- SNP genotyping assays
- CHiP
- Copy number variation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Comment

- Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments

Protocol For TaqMan probe-based real-time PCR analysis of DNA. Superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers
Restrictions For Research Use only
Storage -20 °C
Storage Comment -20°C
Expiry Date 12 months