Binuclease® Nuclease

Details for Product No. ABIN6383963, Supplier: Log in to see
Antigen
  • F15D2.37
  • F15D2_37
  • 5'-3' exonuclease family protein
  • Nuclease
  • AT1G29630
  • Nuc
Reactivity
Serratia marcescens
Host
Yeast (Pichia pastoris)
Antibody Type
Recombinant
Biological Activity
Active
Application
Protein Extraction (PEx)
Options
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Purpose Binuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester.
Brand Binuclease®
Characteristics Recombinant, Benzonase equivalent, From yeast cells with cloned gene encoding genetically engineered Serratia marcescens endonuclease that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. Binuclease is produced from eukaryotic yeast cells that avoid the contamination of endotoxin from prokaryotic cells. The specific activity of Binuclease was meatured at ≥ 1000 kU/mg protein, which is much higher than any existing products (as much as 200%). Binuclease is highly stable under harsh industrial conditions and very cost effective, which make it a perfect alternative for Benzonase.
Purification chromatography
Biological Activity Comment Specific activity: ≥ 1000 kU/mg protein
Unit Definition According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37°C.
Antigen
Molecular Weight ~60 kDa
CAS-No 9025-65-4
Application Notes Cuts All Forms of DNA,RNA, Viscosity Reduction from Protein Solutions, Enhanced Protein Purification, Increased Gel Resolution, Prevention of Cell Clumping
Comment

Activators: 1~10 mM Mg2+

Protocol Binuclease is an ideal tool to remove nucleic acids (DNA and RNA) in biological products or reduce viscosity in cell lysates. Endonuclease is widely used in the pharmaceutical industry to eliminate nucleic acid contamination in vaccine and protein products.
Restrictions For Research Use only
Format Lyophilized
Reconstitution 20 mM Tris-Cl ( pH 8.0), 2 mM MgCl2, 20 mM NaCl
Storage 4 °C
Storage Comment Buffered aqueous glycerol solution: transport with blue ice (below 4°C ). Storage Recommended at -20°C
lyophilized powder: transport at room temperature. Storage Recommended below 4°C.
Storage buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl, 50% glycerol.
Dilution buffer: 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl.
Supplier Images
SDS-PAGE (SDS) image for Binuclease® Nuclease (ABIN6383963) Purity Assay: SDS-PAGE and laser scan analysis of Binuclease. Lane 1-4: 1μg, 2μg, 3μg...
SDS-PAGE (SDS) image for Binuclease® Nuclease (ABIN6383963) Protease Contamination Assay: Samples were incubated with protein marker at 37°C for ...