5mC Tet1 Oxidation Kit for PacBio RS instrument

Details for Product No. ABIN1741994,
DNA Sequencing (Seq), Tet-assisted Bisulfite Sequencing (TAB-Seq)
Purpose This 5mC Tet1 Oxidation Kit is specifically designed to be used to convert 5-methylcytosine (5mC) in genomic DNA to 5-carboxylcytosine (5caC) for subsequent sequencing applications on the PacBio® RS instrument.
Brand PacBio®
Characteristics Each kit contains enough reagents for up to 3 genome-wide or 9 loci-specific sequencing reactions based on recommended protocols.
Components 4 x 1.5 mL tube
Restrictions For Research Use only
Handling Advice Tet1 protein is sensitive to temperature, multiple times of freeze and thaw should be avoided.
Storage -80 °C
Product cited in: Lister, Mukamel, Nery, Urich, Puddifoot, Johnson, Lucero, Huang, Dwork, Schultz, Yu, Tonti-Filippini, Heyn, Hu, Wu, Rao, Esteller, He, Haghighi, Sejnowski, Behrens, Ecker: "Global epigenomic reconfiguration during mammalian brain development." in: Science (New York, N.Y.), Vol. 341, Issue 6146, pp. 1237905, 2013 (PubMed).

Jiang, Zhang, Wang, Wang, Zhang, Li, Yang, Ma, Sun, Cai, Zhang, Huang, Yu, Wang, Liu, Wu, He, Zhang, Ci, Liu: "Sperm, but not oocyte, DNA methylome is inherited by zebrafish early embryos." in: Cell, Vol. 153, Issue 4, pp. 773-84, 2013 (PubMed).

Yu, Hon, Szulwach, Song, Zhang, Kim, Li, Dai, Shen, Park, Min, Jin, Ren, He: "Base-resolution analysis of 5-hydroxymethylcytosine in the mammalian genome." in: Cell, Vol. 149, Issue 6, pp. 1368-80, 2012 (PubMed).