Parodontitis PCR Assay

Details for Product No. ABIN2648723,
Polymerase Chain Reaction (PCR)
Purpose MutaGEL® Parodontitis PCR Assay kit allows the detection of a selection of up to three from the five major pathogens (Porphyromonas gingivalis, Tannerella forsythensis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Prevotella intermedia) leading to chronic parodontitis,agressive juvenile parodontitis and necrotic ulcerative gingivitis. Matrix for this analysis is bacteria-DNA isolated from gingival pocket.
Brand MutaGEL®
Sample Type DNA
Characteristics Parodontitis is the main cause of tooh loss in adults. The mucous membranes of healthy human cavity of the mouth are colonized with billions of microorganisms. The different species (about 300) secrete growth factors and inhibitors thereby regulating their own number. This ecological system of microorganisms is in permanent steady state. Beside the physiological bacteria also pathogenic organisms will grow up in the mouth (more precisely in the gingival pocket). An excessive growth of these pathogens disturbs the physiological balance and favours development of parodontitis (making a therapy with antibiotics necessary). Once started, parodontitis leads in its further course to slow degradation of the parodontium. This stage begins mostly at the age of 30-35 years resulting in first teeth loss at the age of 40 years (if no treatment occurs).

General diagnostic questions are: A) chronic parodontitits (Porphyromonas gingivalis, Tannerella forsythensis and also Fusobacterium necrotum), B) aggressive parodontitis in younger patients (Actinobacillus actinomycetemcomitans) + C) necrotic ulcerative ginigivitis (Prevotella intermedia).

MutaGEL® Parodontitis kit contains different sets of primer which amplify each a specific sequence of the following pathogens: Porphyromonas gingivalis, Tannerella forsythensis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Prevotella intermedia. Additionally, with primers for a highly conserved region of the bacterial 16S-RNA gene, an optical quantification of all contained bacteria can be done (analogous to the classical determination of germ numbers). In dependence of the diagnostic question the required primers for each individual case must be chosen before performance (provided Master Mix is enough to perform up to 96 PCRs and each pathogen specific primer mix allows 24 PCRs). Subsequently, the amplified pathogens are detected by classical gel electrophoresis (Pipetted in parallel. The generated amplicons have all different sizes). Development of this PCR method by Dr. Essrich, Biologisches Labor Freiburg/ Denzlingen (Germany).

Sample Volume 200 μL
Restrictions For Research Use only
Storage -20 °C