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MutaGEL® Parodontitis kit contains different sets of primer which amplify each a specific sequence of the following pathogens: Porphyromonas gingivalis, Tannerella forsythensis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Prevotella intermedia. Additionally, with primers for a highly conserved region of the bacterial 16S-RNA gene, an optical quantification of all contained bacteria can be done (analogous to the classical determination of germ numbers). In dependence of the diagnostic question the required primers for each individual case must be chosen before performance (provided Master Mix is enough to perform up to 96 PCRs and each pathogen specific primer mix allows 24 PCRs). Subsequently, the amplified pathogens are detected by classical gel electrophoresis (Pipetted in parallel. The generated amplicons have all different sizes). Development of this PCR method by Dr. Essrich, Biologisches Labor Freiburg/ Denzlingen (Germany).