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MutaGEL® HLA-B27 PCR Assay Kit contains reagents for two types of DNA amplification: Test A is a “surveying” approach, which can detect all known sequences of the HLA-B27 group contained in a human probe (= types *2701 - *2745). On the other hand Test B gives positive results with nearly all known M. B. associated B27- types, especially again alleles *2705 and *2702, who together asymptotically add to 100 % in Middle European -B27 carriers and M. B. patients. Test B additionally sees allele *2704 from M. B. cases of Asia, whereas the presumably protectives *2706 and famous *2709 are excluded from its amplification.Therefore Test A should be done for the general HLA-B27 search and Test B for the analysis of existence of alleles associated with HLA-B27 disease (of course the alleles seen by Test B are all detected by Test A also and direct comparison allows for conclusion on protective alleles).