One-Step RT-PCR Kit

Details for Product No. ABIN4219213,
Quantitative real-time PCR (qPCR)
Purpose One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification in a single reaction tube. Specifically, the One-Step RT-PCR Kit combines EasyScriptTM RTase and BestaqTM DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR with any RNA template.
Specificity With One-Step RT-PCR Kit, you can streamline the procedure for reverse transcription and subsequent PCR in the same reaction tube, eliminating the need to change buffers and reducing the risk of contamination. This kit offers the end-user an efficient and easy alternative to the conventional "two-step" RT-PCR approach with extreme sensitivity, specificity and high product yield.
G174-dye buffer contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.
Characteristics Our proprietary RT-PCR Buffer contains stabilizers and enhancers that optimize the two reactions into a "single step". Together with our specially formulated RT-PCR Buffer, this One-Step RT-PCR Kit offers endusers an efficient, easy to use, and reliable alternative to conventional "twostep" sequential RT-PCR.
Application Notes - Gene-expression analysis
- Transcription analysis
- Gene cloning
- Multiplex RT-PCR
- High throughput applications
- Virus detection and quantification

100 x 50 μl reactions
- Multiple freezing and thawing of RNA should be avoided. Thaw and keep RNA on ice.
- It is recommended that the One-Step RT-PCR is setup under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% DEPC.
- Wearing gloves when performing procedure is highly recommended.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C.