EasyScript™ cDNA Synthesis Kit

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Application
cDNA Synthesis (cDNA)
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Purpose cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization.
Brand EasyScript™
Specificity - Maximal flexibility in priming - oligo(dT), random primers or gene-specific primers
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
- This kit employs abm's EasyScript™ Reverse Transcriptase.
Characteristics cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.
Components cDNA Synthesis Kit contains all the materials required for first-strand cDNA synthesis, with the choice of using either Oligo (dT) and/or Random Primers. The Oligo (dT) anneals selectively to the poly (A) tail of mRNAs. Random Primers do not require the presence of poly (A) and can be used for the transcription of mRNA 5'-end regions.
Application Notes - Synthesizing cDNA from ssRNA
- DNA primer extension
- Sequencing dsDNA
- Constructing cDNA library
- Producing template for use in RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization
Comment

25 x 20 μl reactions
Restrictions For Research Use only
Buffer In storage buffer containing 50 % glycerol.
Storage -20 °C
Storage Comment Store all components at -20°C.
Product cited in: Monaco, Grimaldi, Ferrandino: "Neuroglial alterations in the zebrafish brain exposed to cadmium chloride." in: Journal of applied toxicology : JAT, Vol. 36, Issue 12, pp. 1629-1638, 2016 (PubMed).

Park, Ju, Park, Song, Shin, Lee, Jeong, Yu, Lee, Cho, Kim, Kim, Kim, Park, Chun: "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity." in: Cell death and differentiation, Vol. 23, Issue 8, pp. 1296-311, 2016 (PubMed).

Wood, Cox, Phelps, Lai, Poddar, Talbot, Mu: "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome." in: Scientific reports, Vol. 6, pp. 19857, 2016 (PubMed).

Akcılar, Emel Koçak, Şimşek, Akcılar, Bayat, Ece, Kökdaşgil: "The effect of adropin on lipid and glucose metabolism in rats with hyperlipidemia." in: Iranian journal of basic medical sciences, Vol. 19, Issue 3, pp. 245-51, 2016 (PubMed).