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25 reactionsTwo-Step RT-PCR kits provide all the necessary reagents required for reverse transcription and PCR amplification in two separate steps. The kit offers the flexibility of optimizing different RT-PCR applications with the choice of either EasyScriptTM or EasyScript PlusTM. The EasyScript PlusTM makes it possible to efficiently synthesize first-strand cDNA from RNA templates with secondary structures and high GC content.