Total-Transcriptome cDNA Synthesis Kit

Details for Product No. ABIN4219235,
cDNA Synthesis (cDNA)
Purpose A complete RNA quantification by revealing all the different RNAs in a sample.
Specificity - Revealing all the RNAs in a human or mouse RNA sample
- A complete, comprehensive cDNA synthesis kit
- Including oligo dT adaptor and Universal reverse primer for high efficiency
- Including positive control assays for mRNA, miRNA, and lncRNA studies
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
Characteristics Total-Transcriptome cDNA Synthesis kit provides all reagents necessary to generate high quality cDNA from all different kind of RNA in a sample such as mRNA, miRNA, and lncRNA. This is made possible by 3' poly(A)-tailing of RNAs via the use of poly(A) polymerase. Oligo d(T) adaptor and random primers then anneal onto the RNA template and facilitate the reverse transcription. EasyScript Plus™ Reverse Transcriptase is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. The EasyScript Plus™ Reverse Transcriptase is engineered to perform under high temperatures (50 °C - 55 °C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. The recombinant RNaseOFF Ribonuclease Inhibitor effectively protects RNA templates from degradation. Also the universal 3' reverse primer included in the kit can be coupled with any specific forward primer in downstream qPCR/PCR amplification.
Application Notes - RNA quantification
- miRNA detection and analysis
- Long non-coding RNA detection and analysis
- Synthesizing cDNA from ssRNA
- Constructing cDNA library
- Sequencing dsDNA
- DNA primer extension
- Producing template for use in RT-PCR
- Labelling 3'-end of duplex DNA via end-filling reactions
- Generating probes for hybridization

25 reactions

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C.