HB_ I RT Reaction kit

Details for Product No. ABIN5326749,
Quantitative real-time PCR (qPCR)
Purpose miR Multi Assay with System I: Detection of DNA amplicon with SYBR Green
Specificity HB miRNA Multi Assay provides...
- Biomarker-specific multiple analysis kit: Reverse-Transcription qPCR analysis kit
- High reactivity: due to very short oligomers (~20mer)
- High accuracy: Multiple verification during Real-time PCR process
- High competency in clinical research: being able to do simultaneous Multiple analysis of miRNA
Components HB_ I RT Primer
HB_ I RT buffer
Reverse Transcriptase
Poly A polymerase
Material not included RNase free water
Background MiRNA is...
- a small non-coding RNA containing ~22 nucleotides
- a negatively regulating gene expression by binding to a specific mRNA and its cleavage
- a regulator for genes related with cancer, cardiovascular and neurologie disease and insulin secretion
- in particular, regarded as a critical regulator for cancer progression
- a novel biomarker for early diagnosis for cancer, customized medicine diagnosis and prognosis prediction etc.
Application Notes System I

microRNA cDNA library synthesis

Assay Time 1 h
Sample Preparation

RNA extraction

Assay Procedure

Reverse transcription at 37 °C for 60 min
Inactivation at 95 °C for 5 min

Calculation of Results

Number of cycles (Ct value) in qPCR

Restrictions For Research Use only
Storage -20 °C