HB_ II Real-Time PCR Master mix kit

Details for Product No. ABIN5326754,
Quantitative real-time PCR (qPCR)
Purpose miR Multi Assay with System II: Detection of specific miRNA with dual-labeled probe
Specificity HB miRNA Multi Assay provides...
- Biomarker-specific multiple analysis kit: Reverse-Transcription qPCR analysis kit
- High reactivity: due to very short oligomers (~20mer)
- High accuracy: Multiple verification during Real-time PCR process
- High competency in clinical research: being able to do simultaneous Multiple analysis of miRNA
Components HB_ II Universal Primer
HB_ II SYBR Green Master mix
Material not included RNase free water
Background MiRNA is...
- a small non-coding RNA containing ~22 nucleotides
- a negatively regulating gene expression by binding to a specific mRNA and its cleavage
- a regulator for genes related with cancer, cardiovascular and neurologie disease and insulin secretion
- in particular, regarded as a critical regulator for cancer progression
- a novel biomarker for early diagnosis for cancer, customized medicine diagnosis and prognosis prediction etc.
Application Notes System II

Quantification of miRNA

Assay Time 1.5 h
Sample Preparation

RNA extraction

Assay Procedure

Initial activation at 65 °C for 15 min
Denaturation at 95 °C for10 sec
Annealing/Extension at 60 °C for 40 sec
40 times cycling from denaturation to extension

Calculation of Results

Number of cycles (Ct value) in qPCR

Restrictions For Research Use only
Storage -20 °C