Magnetic Beads Viral DNA/RNA Kit

Details for Product No. ABIN5526560,
Application
DNA Extraction (DEx), RNA Extraction (REx)
Options
Purpose For efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures
Sample Type Cell Culture Supernatant, Plasma, Serum
Characteristics Features and Benefits:
  • High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Magnetic Bead Concentration: 50 mg/mL
  • Magnetic Bead Size: ~ 5 μm
  • Manual or automated DNA/RNA isolation
  • Operation time: within 30 minutes (manual)
Components MV1 Buffer, MV2 Buffer* (Add Ethanol), MV3 Buffer, MV4 Buffer** (Add Isopropanol), RNAse-free water, MV Magnetic Beads, Carrier RNA*** (Add RNAse Fee water)
Material not included
  • Pipettes; Pipette tips
  • Absolute ethanol
  • ddH2O (RNAse/DNAse free)
  • Microcentrifuge Tubes
  • Magnetic Separator
  • Phosphate-Buffered Saline (PBS)
Application Notes The Magnetic Beads Viral DNA/RNA Kit was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. Viral DNA/RNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Viral DNA/RNA Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA/RNA can be used in qPCR and qRT-PCR assays.
Comment

RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)

Reagent Preparation
  • Vortex magnetic beads to ensure they are in suspension prior to initial use.
  • Add Ethanol (see the bottle label for volume) to to the MV2 and MV4 Buffers and mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.
  • Add 1 ml of RNase-free Water to Carrier RNA then vortex to ensure Carrier RNA is completely dissolved to obtain a working solution of 1 μg/μl. Once it is dissolved completely, centrifuge for a few seconds to spin the mixture down. Divide the Carrier RNA solution into convenient volumes in several RNase-free 1.5 ml microcentrifuge tubes and store at -20ºC. Do not freeze and thaw Carrier RNA solution more than 3 times.
Assay Procedure
  • Transfer a 200 µl sample (e.g. serum, plasma, body fluids or the supernatant of a viral infected cell culture) to a 1.5 ml RNAsefree microcentrifuge tube. NOTE: If the prepared sample is less than 200 µl, adjust the sample volume to 200 µl with PBS. For RNA virus, adding 10 µl of Carrier RNA solution to the sample before extraction is recommended.
  • Add 400 µl of MV1 Buffer to the sample then mix by vortex. Incubate at room temperature for 10 minutes.
  • Add 450 µl of MV2 Buffer (make sure ethanol was added) to the sample lysate then mix by vortex. Vortex MV Magnetic Beads for 10 seconds to ensure the beads are kept in suspension before use. Add 50 µl of MV Magnetic beads to the sample lysate then gently shake the tube for 5 minutes to mix. Be sure the MV Magnetic Beads are dispersed completely in the sample mixture. Place the tube in a magnetic separator for 30 seconds or until MV Magnetic Beads have pelleted then remove and discard the supernatant.
  • Add 400 µl of MV3 Buffer then gently shake the tube for 1 minute. Place the tube in a magnetic separator for 30 seconds or until MV Magnetic Beads have pelleted then remove and discard the cleared supernatant.
  • Add 600 μl of MV4 Buffer (make sure ethanol was added) then gently shake the tube for 1 minute. Place the tube in a magnetic separator for 30 seconds or until MV Magnetic beads have pelleted then remove and discard the supernatant. Once again, add 600 μl of MV4 Buffer (make sure ethanol was added) then gently shake the tube for 1 minute. Place the tube in a magnetic separator for 30 seconds or until MV Magnetic Beads have pelleted then remove and discard the supernatant.
  • Incubate the tube on a 40ºC hot plate for 3 minutes to dry the MV Magnetic Beads. Add 50–100 µl of RNAse-free Water then mix the sample by pipetting and incubate the sample at room temperature for 3 minutes. During incubation, keep the MV Magnetic Beads in suspension by mixing. Place the tube in a magnetic separator for 30 seconds or until MV Magnetic Beads have pelleted. Transfer the supernatant containing the purified Viral DNA/RNA to a RNAse-free 1.5 ml microcentrifuge tube.
Restrictions For Research Use only
Storage RT/4 °C/-20 °C
Storage Comment All the reagents are shipped and Stored at room temperature (15-25ºC). All the reagents are stored at room temperature (15-25ºC) except the carrier RNA for up to 12 months without showing any reduction in performance. The Carrier RNA in Water solution should be stored at - 20ºC. Do not freeze and thaw Carrier RNA solution more than 3 times.
Expiry Date 12 months