Fast-Fusion Cloning Kit

Details for Product No. ABIN5666473, Supplier: Log in to see
Application
Cloning (Clon)
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Purpose The Fast-Fusion Cloning Kit is designed for fast, directional cloning of DNA fragment into any vector at any site. It can simplify and accelerate your experiments without requiring PCR product cutting with restriction enzymes allowing flexible options for restriction enzyme sites when linearizing the vector.
Characteristics The Fast-Fusion Cloning Kit is designed for fast, directional cloning of DNA fragment into any vector at any site. It can simplify and accelerate your experiments without requiring PCR product cutting with restriction enzymes allowing flexible options for restriction enzyme sites when linearizing the vector. Additionally, the linearized vector and PCR product can be cyclized in only 5 minutes at 70 °C. Compared to the conventional cloning method, the 15 base tract is added at the ends of PCR primers corresponding to the ends of the linearized vector.
Components Fast-Fusion Enzyme, 10xFast-Fusion Buffer
Application Notes PCR cloning into any vector, Gene transfer from one vector to another, Add adaptor, linker and tag before or after the inset, High throughput cloning
Restrictions For Research Use only