First Chain cDNA Synthesis Kit

Details for Product No. ABIN5666475, Supplier: Log in to see
Application
cDNA Synthesis (cDNA)
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Purpose The First Strand cDNA Synthesis Kit for RT-PCR is optimized to efficiently synthesize first cDNA from mRNA or total RNA templates.
Characteristics The First Strand cDNA Synthesis Kit for RT-PCR is optimized to efficiently synthesize first chain cDNA from mRNA or total RNA templates. The kit uses M-MLV reverse transcriptase with lower RNase H activity, which avoids degradation of RNA when RNA-DNA hybrid forms in the progress of cDNA synthesis. It is suitable for synthesis of cDNA up to 9 kb. The cDNA products could be used directly in a variety of downstream applications, including PCR, qPCR, gene clone.
Components M-MLV (200 U/ μL), OligodT primer, Random primer, 5 x Reaction buffer, dNTP mix (10 mM each), RNase Inhibitor, RNase-free H2O
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Handling Advice Avoid repeated freeze-thaw cycle
Storage -20 °C
Storage Comment Store all system components at -20°C for one year.