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The Plasmid Minipred Kit could obtain 5-30 μg pure plasmids DNA from 1-4 ml culture. And the ratio of OD260/OD280 is generally between 1.8 and 2.0. Plasmid DNA prepared using the Plasmid Minipred Kit is suitable for a variety of routine applications including PCR, enzyme digestion, sequencing, in vitro transcription, ligation and transformation.