RT Reagent Kit (for qPCR)

Details for Product No. ABIN5666480,
Application
Quantitative real-time PCR (qPCR)
Options
Purpose The RT reagent Kit is designed for the first step of RT-PCR. TonkBio™ M-MLV with high extension ability can complete cDNA synthesis in 15 minutes.
Characteristics The RT reagent Kit is designed for the first step of RT-PCR. M-MLV with high extension ability can complete cDNA synthesis in 15 minutes. This kit is suitable for high throughput analysis because its easy operation and short time consumed.

The RT reagent Kit supplies two kinds primers, the OligodT primer and Random primer. Choose correct primers according to specific reaction. The components of this kit have high compatibility with qPCR.
Components - RT Enzyme Mix 100 μL
- OligodT primer (50 μM) 100 μL
- Random primer (100 μM) 400 μL
- 5 x Reaction buffer (for qPCR) 400 μL
- RNase-free H2O 1.2 mL
RT Enzyme Mix contains RNase inhibitor.
5 x Reaction buffer contains dNTP and Mg2+.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Handling Advice Avoid repeated freeze-thaw cycle
Storage -20 °C
Storage Comment Store all system components at -20°C for one year.