Easy™ One Step RT Kit-Dye

Details for Product No. ABIN5684252,
Real Time PCR (rtPCR)
Purpose One Step RT PCR Kit
Characteristics EasyTM One Step RT Kit contains all the necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One Step RT PCR kit contains EvoTM Reverse Transcriptase and DistantTM DNA Polymerase in a convenient format for highly sensitive and specific RT PCR using any RNA template. Our proprietary RT PCR buffer contains stabilizers and enhancers that optimize the two reactions in a ""single step"". Together with a specially formulated RT PCR buffer, this One-Step RT PCR kit offers the endusers an efficient, easy to use and reliable alternative to the conventional ""two-step"" sequential RT PCR. The dye buffer contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.
EasyTM One Step RT PCR Kit offers the end-user an efficient and easy alternative to the conventional ""two step"" RT PCR approach with extreme sensitivity, specificity and high product yield.
Components DistantTM DNA Polymerase (5 U/μl), EvoTM RTase (200 U/μl), 2X One Step RT PCR Buffer with Dye
Application Notes - Gene-expression analysis
- Transcription analysis
- Gene cloning
- Multiplex RT PCR
- High throughput applications
- Virus detection and quantification

One Step RT PCR Kit

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly. Avoid repeated freeze-thaw cycles to retain maximum performance. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months