Evo™ Two Step RT Kit

Details for Product No. ABIN5684253, Supplier: Log in to see
Application
Real Time PCR (rtPCR)
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Purpose EvoTM Two Step RT Kit is a highly efficient two step RT PCR kit that consists of two main components: EvoTM cDNA Supermix and Robust ReadyTM 2X PCR Master Mix with dye.
Components EvoTM RTase (200 U/μl), 2X cDNA Synthesis Reaction Mix, Nuclease-free H2O, Robust ReadyTM 2X PCR Master Mix-Dye
Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of deoxynucleotide into acid-precipitable material in 10 min at 37°C using poly(A) and Oligo(dT) as template and primer respectively.
Application Notes - Routine RT PCR amplification of RNA templates
- Reliable RT PCR based detection of gene expression
Comment

- Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yields and robust PCR performance
- EvoTM Two Step RT PCR Kit is the most convenient, efficient and time saving two step RT PCR kit.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly. Avoid repeated freeze-thaw cycles to retain maximum performance. Briefly centrifuge small vials prior to opening.
Expiry Date 12 months