DNA Quantification Assay Kit (Fluorometric)

Details for Product No. ABIN6933397,
Detection Method
Fluorometric
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Purpose Quantification of DNA for rtPCR, qPCR, Next Generation Sequencing (NGS)
Sample Type DNA samples
Detection Method Fluorometric
Characteristics This DNA Quantification Assay Kit provides a quick, specific, and easy method for the measurement of DNA concentrations in a wide variety of samples. In this assay, DNA specifically reacts with a probe producing a stable fluorescence signal (Ex/Em = 492/528 nm). The kit is simple to perform, specific, sensitive and high-throughput adaptable with a wide detection range (0.4-150 ng DNA) and a limit of detection of 4 pg/μl dsDNA in samples.

Features and Benefits: Simple, one-step, results in 30 min or less,Sensitive, specific,Does not detect protein, RNA or ssDNA
Components 200X DNA Probe (in DMSO),λDNA Standard (3 ng/μl),10X DNA Buffer
Background DNA quantification is essential for a variety of applications, such as real-time PCR (rtPCR), qPCR, Next Generation Sequencing (NGS), etc. The measurement of DNA using analytical instruments (i.e. ""nanodrop"") is often limited by the presence/contamination of other macromolecules like protein and RNAs found in biological samples. However, the development of highly specific probes that bind to DNA allows the measurement of this macromolecule using a fluorimeter. The biggest advantages of this approach is protein, RNA and single-stranded DNA (ssDNA) are not detected.
Comment

Further details regarding sample type: Purified DNA samples

Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months