DNA Quantification Assay Kit (Fluorometric)

Details for Product No. ABIN6933397,
Detection Method
Purpose Quantification of DNA for rtPCR, qPCR, Next Generation Sequencing (NGS)
Sample Type DNA samples
Detection Method Fluorometric
Characteristics This DNA Quantification Assay Kit provides a quick, specific, and easy method for the measurement of DNA concentrations in a wide variety of samples. In this assay, DNA specifically reacts with a probe producing a stable fluorescence signal (Ex/Em = 492/528 nm). The kit is simple to perform, specific, sensitive and high-throughput adaptable with a wide detection range (0.4-150 ng DNA) and a limit of detection of 4 pg/μl dsDNA in samples.

Features and Benefits: Simple, one-step, results in 30 min or less,Sensitive, specific,Does not detect protein, RNA or ssDNA
Components 200X DNA Probe (in DMSO),λDNA Standard (3 ng/μl),10X DNA Buffer
Background DNA quantification is essential for a variety of applications, such as real-time PCR (rtPCR), qPCR, Next Generation Sequencing (NGS), etc. The measurement of DNA using analytical instruments (i.e. ""nanodrop"") is often limited by the presence/contamination of other macromolecules like protein and RNAs found in biological samples. However, the development of highly specific probes that bind to DNA allows the measurement of this macromolecule using a fluorimeter. The biggest advantages of this approach is protein, RNA and single-stranded DNA (ssDNA) are not detected.

Further details regarding sample type: Purified DNA samples

Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months