EZClick™ TUNEL - in situ DNA Fragmentation/Apoptosis Assay Kit

Details for Product No. ABIN6933400,
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Purpose Detection of DNA fragmentation during apoptosis and programmed cell death,Evaluating effects of anti-cancer drugs and genotoxic agents on DNA structure,Screening for genotoxic compounds and effectors of DNA integrity and cellular changes,In-situ studies of apoptosis and programmed cell death
Brand EZClick™
Sample Type Cell Culture Cells
Characteristics This EZClick™ TUNEL-DNA fragmentation/Apoptosis Assay utilizes modified EdUTP nucleotides which are incorporated at the 3'-OH ends of fragmented DNA by Terminal deoxynucleotidyl transferase (TdT) enzyme and detected based on a click reaction. This assay enables easy delivery and incorporation of EdUTP coupled with high selectivity of detection of DNA damage. The assay is performed under mild reaction conditions which preserve cell morphology, thus enabling the identification of damaged DNA by FACS or fluorescence microscopy. Our assay is fast and capable of detecting a higher percentage of apoptotic cells than the antibody based methods. The kit contains sufficient reagents to detect total/fragmented DNA in apoptotic cells in a 1 X 96-well plate or on 50 cover slips.

Features and Benefits: Does not require antibodies, avoiding lengthy incubation times and does not require washes,Specific, sensitive,Compatible with Flow Cytometry and Fluorescence Microscopy instruments
Components EZClickTM Total DNA Stain (1000X),EZClickTM Wash Buffer (10X),Permeabilization Buffer (10X),Reducing Agent (20X),EZClickTM Fluorescent Azide (100X),Fixative Solution,EZClickTM EdUTP DNA Label (50X),TUNEL Enzyme Buffer,EZClickTM TUNEL Reaction Buffer (10X),EZClickTM TUNEL Enzyme,Copper Reagent (100X)
Background Apoptosis is a controlled phenomenon in which apoptotic cells are characterized by changes in nucleus (i.e. chromatin condensation, DNA fragmentation), cytoplasm shrinkage and production of apoptotic bodies. These are subsequently phagocytosed by macrophages, parenchymal cells, or neoplastic cells. DNA strand breaks can be analyzed by TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End-Labeling) assays.
Comment

Further details regarding sample type: Cell culture

Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months