Coronavirus (SARS-CoV-2) Real Time RT-PCR Nucleic Acid Detection Kit

Details for Product No. ABIN6952468,
Target
  • nucleocapsid phosphoprotein
  • N
Reactivity
SARS Coronavirus-2 (SARS-CoV-2)
Application
Real Time PCR (rtPCR)
Options
Purpose Suitable for the qualitative detection of new coronavirus (SARS-CoV-2 / COVID-19) nucleocapsid protein N gene in human throat swabs and alveolar lavage. Includes a one-step RT-qPCR mix.
Sample Type Bronchoalveolar Lavage, Throat Swab
Analytical Method Qualitative
Characteristics The Coronavirus (SARS-CoV-2) Real Time RT-PCR Nucleic Acid Detection Kit is based on the PCR method which uses a fluorescent probe and a specific primer to detect three specific regions within the novel coronavirus (SARS-CoV-2) nucleocapsid protein N gene. The kit includes 3 primer-probe sets corresponding to those used in the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel.

Primer-probe sets N1 and N2 detect SARS-COV-2 specifically, with no expected false positives from other coronaviruses or human microflora, while N3 detects SARS-COV-2 as well as human SARS coronavirus, but no expected false positives from other coronaviruses or human microflora. The kit also includes a primer-probe set specific to the to the human housekeeping gene, ribonuclease P (RNP), are included to serve as an internal reference to monitor sample collection, RNA extraction, and amplification.

This product is for in vitro diagnostic use, following guidance from the FDA for Emergency Use Authorizations of tests submitted for approval on March 16, 2020. This test has not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status.
Components Primers and Probe N1 Solution (Primers & probe for N1) 140 μL
Primers and Probe N2 Solution (Primers & probe for N2) 140 μL
Primers and Probe N3 Solution (Primers & probe for N3) 140 μL
Primers and Probe RP Solution (Primers & probe for Human RNAase P) 140 μL
PCR Reaction Solution (Buffer, dNTP's) 12000 μL
PCR Enzyme Mix (Reverse transcriptase (RT) enzyme, DNA polymerase) 96 μL
Positive Control (Plasmid DNA containing target gene (Nucleocapsid protein) + plasmid DNA containing internal control gene fragment (RNP)) 480 μL
Negative Control (Nuclease-free water) 480 μL
Material not included
  • Fluorescence qPCR instrument capable of reading FAM or equivalent channels (494 nm maximum absorption, 518 nm maximum emission).
  • Vortex Mixer
  • Microcentrifuge
  • Pipettes
  • Sterile nuclease-free pipette tips and microfuge tubes
  • RNA extraction kit
Target Name (Antigen)
Alternative Name SARS-CoV-2 Nucleocapsid
Background N1, N2, N3, RNP
Application Notes Optimal working dilution should be determined by the investigator.
Sample Preparation

Sample types: total RNA extracts from throat swab or alveolar lavage fluid. Note: collection of samples should be conducted according to the relevant guidelines of your governing body.

Samples should be regarded as a potential source of infection. Sample handling should be performed in a microbiological and biomedical laboratory with a biosafety label to protect the operator from possible exposure during work.

Restrictions For Research Use only
Storage -20 °C
Storage Comment The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for 6 months if stored at -20°C. Up to 3 freeze-thaw cycles are permitted while retaining activity.
Images
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