Mouse SMAD3 CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a SMAD, Mothers Against DPP Homolog 3 plasmid from OriGene with gRNA + Cas9 insert cloned into Mammalian Expression VectorpCas-Guide. Insert length: not_set. Transient expression. Suitable for GEEN.
Bacterial selection: Ampicillin.
1 image
Catalog No. ABIN3223824
Supplier Product No.: kn316272
$1,640.43
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 21 to 22 Business Days
Quick Overview for Mouse SMAD3 CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3223824)
Gene
SMAD3
(SMAD, Mothers Against DPP Homolog 3 (SMAD3))
Fusion tag
Myc-DYKDDDDK Tag
Application
Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Mammalian Expression Vector
Vector Backbone
pCas-Guide
Promoter
U6 Promoter, Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
-
-
Species
- Mouse
-
Supplier Product No.
- kn316272
-
Supplier
- OriGene
-
Purpose
- Knockout Kit for Mouse (Murine) Smad3 via CRISPR.
-
Characteristics
-
- The Smad3 kit is designed based on the best knowledge of CRISPR technology.
- The system has been functionally validated for knocking-in the cassette downstream the native promoter.
- The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
-
Components
-
- Smad3 gRNA vector 1 in pCAS-Guide vector.
- Smad3 gRNA vector 2 in pCAS-Guide vector.
- Donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
- Scramble sequence in pCas-Guide vector
-
Sequencing Primer
- CF3 (ACGATACAAGGCTGTTAGAGAG)
-
-
-
-
Application Notes
-
- Knock-in GFP reporter for promoter study.
- Knock-out genes at chromosomal level.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- -20 °C
-
-
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
-
Alternative Name
- Smad3
Target
-
You are here:
