Human SMIM22 CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
Quick Overview for Human SMIM22 CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3223932)
Gene
Fusion tag
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- kn433242
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Supplier
- OriGene
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Purpose
- Knockout Kit for Human SMIM22 via CRISPR.
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Characteristics
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- The SMIM22 kit is designed based on the best knowledge of CRISPR technology.
- The system has been functionally validated for knocking-in the cassette downstream the native promoter.
- The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
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Components
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- SMIM22 gRNA vector 1 in pCAS-Guide vector.
- SMIM22 gRNA vector 2 in pCAS-Guide vector.
- Donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
- Scramble sequence in pCas-Guide vector
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Sequencing Primer
- CF3 (ACGATACAAGGCTGTTAGAGAG)
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![Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
![Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
![Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
![Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Mouse SMIM22 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)cDNA PExp Mammalian Expression Vector pCMV6-Entry Kanamycin Enhanced CMV Promoter unconjugated Transient
![Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)ORF PExp Mammalian Expression Vector pCMV6-Entry Kanamycin CMV Promoter Myc-DYKDDDDK Tag Transient
![Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)ORF PExp Mammalian Expression Vector pCMV6-Entry Kanamycin CMV Promoter Myc-DYKDDDDK Tag Transient
![Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)ORF PExp Mammalian Expression Vector pCMV6-Entry Kanamycin CMV Promoter Myc-DYKDDDDK Tag Transient
![Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)]()
Human SMIM22 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)ORF PExp Mammalian Expression Vector pCMV6-Entry Kanamycin CMV Promoter Myc-DYKDDDDK Tag Transient
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Application Notes
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- Knock-in GFP reporter for promoter study.
- Knock-out genes at chromosomal level.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- -20 °C
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- SMIM22 (Small Integral Membrane Protein 22 (SMIM22))
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Alternative Name
- SMIM22
Target
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