Mouse NPAT CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
gRNA + Cas9
GEEN
Mammalian Expression Vector
pCas-Guide
Ampicillin
U6 Promoter
Myc-DYKDDDDK Tag
Transient
1 image
Catalog No. ABIN3233520
Supplier Product No.: kn511143
Quick Overview for Mouse NPAT CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3233520)
Gene
NPAT
(Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))
Fusion tag
Myc-DYKDDDDK Tag
Application
Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Mammalian Expression Vector
Vector Backbone
pCas-Guide
Promoter
U6 Promoter, Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Mouse
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Supplier Product No.
- kn511143
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Supplier
- OriGene
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Purpose
- Knockout Kit for Mouse (Murine) Npat via CRISPR.
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Characteristics
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- The Npat kit is designed based on the best knowledge of CRISPR technology.
- The system has been functionally validated for knocking-in the cassette downstream the native promoter.
- The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
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Components
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- Npat gRNA vector 1 in pCAS-Guide vector.
- Npat gRNA vector 2 in pCAS-Guide vector.
- Donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
- Scramble sequence in pCas-Guide vector
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Sequencing Primer
- CF3 (ACGATACAAGGCTGTTAGAGAG)
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Application Notes
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- Knock-in GFP reporter for promoter study.
- Knock-out genes at chromosomal level.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- -20 °C
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- NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))
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Alternative Name
- Npat
Target
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