Human FOXA2 CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
gRNA + Cas9
GEEN
Mammalian Expression Vector
pCas-Guide
Ampicillin
U6 Promoter
Myc-DYKDDDDK Tag
Transient
1 image
(1 reference)
Catalog No. ABIN3235126
Supplier Product No.: kn204066
Quick Overview for Human FOXA2 CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3235126)
Gene
FOXA2
(Forkhead Box A2 (FOXA2))
Fusion tag
Myc-DYKDDDDK Tag
Application
Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Mammalian Expression Vector
Vector Backbone
pCas-Guide
Promoter
U6 Promoter, Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- kn204066
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Supplier
- OriGene
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Purpose
- Knockout Kit for Human FOXA2 via CRISPR.
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Characteristics
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- The FOXA2 kit is designed based on the best knowledge of CRISPR technology.
- The system has been functionally validated for knocking-in the cassette downstream the native promoter.
- The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
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Components
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- FOXA2 gRNA vector 1 in pCAS-Guide vector.
- FOXA2 gRNA vector 2 in pCAS-Guide vector.
- Donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
- Scramble sequence in pCas-Guide vector
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Sequencing Primer
- CF3 (ACGATACAAGGCTGTTAGAGAG)
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Application Notes
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- Knock-in GFP reporter for promoter study.
- Knock-out genes at chromosomal level.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- -20 °C
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: "Transcriptomic and CRISPR/Cas9 technologies reveal FOXA2 as a tumor suppressor gene in pancreatic cancer." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 11, pp. G1124-37, (2016) (PubMed).
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: "Transcriptomic and CRISPR/Cas9 technologies reveal FOXA2 as a tumor suppressor gene in pancreatic cancer." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 11, pp. G1124-37, (2016) (PubMed).
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- FOXA2 (Forkhead Box A2 (FOXA2))
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Alternative Name
- FOXA2
Target
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