Human DOT1L CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) plasmid from OriGene with gRNA + Cas9 insert cloned into Mammalian Expression VectorpCas-Guide. Insert length: not_set. Transient expression. Suitable for GEEN.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3253612
Supplier Product No.: kn418547
$1,640.43
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 21 to 22 Business Days
Quick Overview for Human DOT1L CRISPR gRNA + Cas9 in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3253612)
Gene
DOT1L
(DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
Fusion tag
Myc-DYKDDDDK Tag
Application
Genome Editing with Engineered Nucleases (GEEN)
Insert
gRNA + Cas9
Vector
Mammalian Expression Vector
Vector Backbone
pCas-Guide
Promoter
U6 Promoter, Enhanced CMV Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- kn418547
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Supplier
- OriGene
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Purpose
- Knockout Kit for Human DOT1L via CRISPR.
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Characteristics
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- The DOT1L kit is designed based on the best knowledge of CRISPR technology.
- The system has been functionally validated for knocking-in the cassette downstream the native promoter.
- The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
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Components
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- DOT1L gRNA vector 1 in pCAS-Guide vector.
- DOT1L gRNA vector 2 in pCAS-Guide vector.
- Donor vector containing Left and right homologous arms and GFP-Puro functional cassette.
- Scramble sequence in pCas-Guide vector
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Sequencing Primer
- CF3 (ACGATACAAGGCTGTTAGAGAG)
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Application Notes
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- Knock-in GFP reporter for promoter study.
- Knock-out genes at chromosomal level.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- -20 °C
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- DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
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Alternative Name
- DOT1L
Target
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