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Human RBM23 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)

This is a RNA Binding Motif Protein 23 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: 0 bp. Transient expression. Suitable for PExp. Bacterial selection: Kanamycin.
OriGene
Catalog No. ABIN3304509
Supplier Product No.: sc315556
$497.97
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 21 to 22 Business Days

Quick Overview for Human RBM23 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3304509)

Gene

RBM23 (RNA Binding Motif Protein 23 (RBM23))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-Entry

Promoter

Enhanced CMV Promoter

Bacterial Resistance

Kanamycin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc315556

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human RBM23 is ideal for over-expression of native protein for functional studies.

    Specificity

    With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Insert Length

    0 bp

    Selectable Marker

    Neomycin

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Target

    RBM23 (RNA Binding Motif Protein 23 (RBM23))

    Alternative Name

    RBM23

    Background

    This gene encodes a member of the U2AF-like family of RNA binding proteins. This protein interacts with some steroid nuclear receptors, localizes to the promoter of a steroid- responsive gene, and increases transcription of steroid-responsive transcriptional reporters in a hormone-dependent manner. It is also implicated in the steroid receptor-dependent regulation of alternative splicing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

    NCBI Accession

    NM_001077351, NP_001070819
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