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Human RBMXL1 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)

This is a RNA Binding Motif Protein, X-Linked-Like 1 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp. Bacterial selection: Kanamycin.
OriGene
Catalog No. ABIN3304522
Supplier Product No.: sc327505
$724.68
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 21 to 22 Business Days

Quick Overview for Human RBMXL1 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3304522)

Gene

RBMXL1 (RNA Binding Motif Protein, X-Linked-Like 1 (RBMXL1))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-Entry

Promoter

Enhanced CMV Promoter

Bacterial Resistance

Kanamycin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc327505

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human RBMXL1 is ideal for over-expression of native protein for functional studies.

    Specificity

    With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Selectable Marker

    Neomycin

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Target

    RBMXL1 (RNA Binding Motif Protein, X-Linked-Like 1 (RBMXL1))

    Alternative Name

    RBMXL1

    Background

    This gene represents a retrogene of RNA binding motif protein, X-linked (RBMX), which is located on chromosome X. While all introns in the coding sequence have been processed out compared to the RBMX locus, the ORF is intact and there is specific evidence for transcription at this location. The preservation of the ORF by purifying selection in all Old World monkeys carrying it suggests that this locus is likely to be functional, possibly during male meiosis when X chromosomal genes are silenced or during haploid stages of spermatogenesis. This gene shares 5' exon structure with the cysteine conjugate-beta lyase 2 locus on chromosome 1, but the coding sequences are non-overlapping. Alternative splicing results in two transcript variants. [provided by RefSeq, Jun 2009].Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

    NCBI Accession

    NM_001162536, NP_001156008
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