Human RGS3 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a Regulator of G-Protein Signaling 3 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin.
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Catalog No. ABIN3304619
Supplier Product No.: sc304530
$610.83
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human RGS3 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3304619)
Gene
RGS3
(Regulator of G-Protein Signaling 3 (RGS3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc304530
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human RGS3 is ideal for over-expression of native protein for functional studies.
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Specificity
- With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- RGS3 (Regulator of G-Protein Signaling 3 (RGS3))
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Alternative Name
- RGS3
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Background
- This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013].Transcript Variant: This variant (3) lacks several 5' and 3' exons but includes alternate 5' and 3' exons, and it thus differs in both UTRs and in the 5' and 3' coding regions, compared to variant 6. The encoded isoform (3) is shorter and has distinct N- and C-termini, compared to isoform 6.
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NCBI Accession
- NM_017790, NP_060260
Target
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