Human SHBG cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a Sex Hormone Binding Globulin plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin.
1 image
Catalog No. ABIN3305040
Supplier Product No.: sc326864
$326.70
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 21 to 22 Business Days
Quick Overview for Human SHBG cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3305040)
Gene
SHBG
(Sex Hormone Binding Globulin (SHBG))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
-
-
Species
- Human
-
Supplier Product No.
- sc326864
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human SHBG is ideal for over-expression of native protein for functional studies.
-
Specificity
- With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Selectable Marker
- Neomycin
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
- SHBG (Sex Hormone Binding Globulin (SHBG))
-
Alternative Name
- SHBG
-
Background
- This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].Transcript Variant: This variant (3) lacks an alternate exon in the 3' coding region which results in a frameshift compared to variant 1. Isoform 3 is shorter and has a distinct C-terminus compared to isoform 1.
-
NCBI Accession
- NM_001146280, NP_001139752
Target
-
You are here:
