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Human SLC15A2 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)

This is a Solute Carrier Family 15 (H+/Peptide Transporter), Member 2 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp. Bacterial selection: Kanamycin.
OriGene
Catalog No. ABIN3305118
Supplier Product No.: sc327179
$696.96
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 21 to 22 Business Days

Quick Overview for Human SLC15A2 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3305118)

Gene

SLC15A2 (Solute Carrier Family 15 (H+/Peptide Transporter), Member 2 (SLC15A2))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-Entry

Promoter

Enhanced CMV Promoter

Bacterial Resistance

Kanamycin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc327179

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human SLC15A2 is ideal for over-expression of native protein for functional studies.

    Specificity

    With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Selectable Marker

    Neomycin

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Target

    SLC15A2 (Solute Carrier Family 15 (H+/Peptide Transporter), Member 2 (SLC15A2))

    Alternative Name

    SLC15A2

    Background

    The mammalian kidney expresses a proton-coupled peptide transporter that is responsible for the absorption of small peptides, as well as beta-lactam antibiotics and other peptide-like drugs, from the tubular filtrate. This transporter, SLC15A2, belongs to the same gene family as SLC15A1 (MIM 600544), the proton-coupled peptide transporter found in the small intestine (Liu et al, 1995 [PubMed 7756356]).[supplied by OMIM, Feb 2011].Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a.

    NCBI Accession

    NM_001145998, NP_001139470
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