Human SLC2A14 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
Quick Overview for Human SLC2A14 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3305194)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- sc306610
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SLC2A14 is ideal for over-expression of native protein for functional studies.
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Specificity
- With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- SLC2A14 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 14 (SLC2A14))
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Alternative Name
- SLC2A14
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Background
- Members of the glucose transporter (GLUT) family, including SLC2A14, are highly conserved integral membrane proteins that transport hexoses such as glucose and fructose into all mammalian cells. GLUTs show tissue and cell-type specific expression (Wu and Freeze, 2002 [PubMed 12504846]).[supplied by OMIM, Mar 2008].Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (a).
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NCBI Accession
- NM_153449, NP_703150
Target
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