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Human SLC36A4 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)

This is a Solute Carrier Family 36 (Proton/amino Acid Symporter), Member 4 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp. Bacterial selection: Kanamycin.
OriGene
Catalog No. ABIN3305224
Supplier Product No.: sc317847
$511.83
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 21 to 22 Business Days

Quick Overview for Human SLC36A4 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3305224)

Gene

SLC36A4 (Solute Carrier Family 36 (Proton/amino Acid Symporter), Member 4 (SLC36A4))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-Entry

Promoter

Enhanced CMV Promoter

Bacterial Resistance

Kanamycin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc317847

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human SLC36A4 is ideal for over-expression of native protein for functional studies.

    Specificity

    With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Selectable Marker

    Neomycin

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Target

    SLC36A4 (Solute Carrier Family 36 (Proton/amino Acid Symporter), Member 4 (SLC36A4))

    Alternative Name

    SLC36A4

    Background

    SLC36A4 belongs to the SLC36 family of amino acid transporters based on sequence similarity with other family members (e.g., SLC36A1, MIM 606561). SLC36 proteins contain about 500 amino acids and have 9 to 11 transmembrane domains. Unlike other SLC36 family members, which are proton-coupled amino acid transporters, SLC36A4 is a high-affinity/low-capacity non-proton-coupled amino acid transporter (Pillai and Meredith, 2011 [PubMed 21097500]).[supplied by OMIM, Feb 2011].Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).

    NCBI Accession

    NM_152313, NP_689526
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