Human U11/U12 SnRNP 35 KDa Protein cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a U11/U12 SnRNP 35 KDa Protein plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin.
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Catalog No. ABIN3305366
Supplier Product No.: sc307238
$326.70
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human U11/U12 SnRNP 35 KDa Protein cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3305366)
Gene
SNRNP35
(U11/U12 SnRNP 35 KDa Protein)
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc307238
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SNRNP35 is ideal for over-expression of native protein for functional studies.
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Specificity
- With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- SNRNP35 (U11/U12 SnRNP 35 KDa Protein)
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Alternative Name
- SNRNP35
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Background
- The protein encoded by this gene is a homolog of the U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal half is rich in Arg/Asp and Arg/Glu dipeptides, which is a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. Alternative splicing results in multiple transcript variants encoding two distinct isoforms and representing a non-protein coding variant. [provided by RefSeq, Aug 2013].Transcript Variant: This variant (3) includes an alternate exon and initiates translation at an alternate start codon, compared to variant 2. The full extent of the 5' UTR of this variant has not been determined. The encoded isoform (b) has a longer N-terminus, compared to isoform a.
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NCBI Accession
- NM_180699, NP_851030
Target
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