Human SOX6 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a SRY (Sex Determining Region Y)-Box 6 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin.
1 image
(1 reference)
Catalog No. ABIN3305400
Supplier Product No.: sc304470
$1,093.95
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human SOX6 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3305400)
Gene
SOX6
(SRY (Sex Determining Region Y)-Box 6 (SOX6))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
-
-
Species
- Human
-
Supplier Product No.
- sc304470
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human SOX6 is ideal for over-expression of native protein for functional studies.
-
Specificity
- With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Selectable Marker
- Neomycin
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
-
: "Osteochondral tissue regeneration through polymeric delivery of DNA encoding for the SOX trio and RUNX2." in: Acta biomaterialia, Vol. 10, Issue 10, pp. 4103-12, (2014) (PubMed).
-
-
- SOX6 (SRY (Sex Determining Region Y)-Box 6 (SOX6))
-
Alternative Name
- SOX6
-
Background
- This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009].Transcript Variant: This variant (1) represents the longest transcript.
-
NCBI Accession
- NM_017508, NP_059978
Target
-
You are here:
