Human TFAP2A cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin.
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Catalog No. ABIN3305905
Supplier Product No.: sc311273
$497.97
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 21 to 22 Business Days
Quick Overview for Human TFAP2A cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3305905)
Gene
TFAP2A
(Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc311273
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TFAP2A is ideal for over-expression of native protein for functional studies.
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Specificity
- With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The predominant WT1 isoform (+KTS) encodes a DNA-binding protein targeting the planar cell polarity gene Scribble in renal podocytes." in: Molecular cancer research : MCR, Vol. 8, Issue 7, pp. 975-85, (2010) (PubMed).
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- TFAP2A (Transcription Factor AP-2 alpha (Activating Enhancer Binding Protein 2 Alpha) (TFAP2A))
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Alternative Name
- TFAP2A
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Background
- The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009].Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) has a shorter and distinct N-terminus compared to isoform a.
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NCBI Accession
- NM_001042425, NP_001035890
Target
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