Human TMED7 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a Transmembrane Emp24 Protein Transport Domain Containing 7 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin.
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Catalog No. ABIN3306003
Supplier Product No.: sc326959
$497.97
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TMED7 cDNA Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN3306003)
Gene
TMED7
(Transmembrane Emp24 Protein Transport Domain Containing 7 (TMED7))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
Enhanced CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc326959
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TMED7 is ideal for over-expression of native protein for functional studies.
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Specificity
- With the native stop codon at the end of the ORF the C-terminal Myc-DDK tag in the vector won't be expressed.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Selectable Marker
- Neomycin
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- TMED7 (Transmembrane Emp24 Protein Transport Domain Containing 7 (TMED7))
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Alternative Name
- TMED7
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Background
- This locus represents naturally occurring read-through transcription between the neighboring transmembrane emp24 protein transport domain containing 7 (TMED7) and toll-like receptor adaptor molecule 2 (TICAM2) genes. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. This fusion product functions to negatively regulate the adaptor MyD88-independent toll-like receptor 4 pathway. [provided by RefSeq, Nov 2010].Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1), also known as TAG. This isoform shares sequence identity with the products of both the TMED7 and TICAM2 genes.
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NCBI Accession
- NM_001164468, NP_001157940
Target
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