Human COX4I1 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
Quick Overview for Human COX4I1 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN5342524)
Gene
Fusion tag
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- rc209374
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Supplier
- OriGene
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Purpose
- Mammalian Vector with ORF clone of Human cytochrome c oxidase subunit IV isoform 1 (COX4I1)
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Specificity
- Restriction Site: SgfI-MluI
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Grade
- End-sequenced
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Components
- The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
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Insert Length
- 510 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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- COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))
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Background
- Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
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NCBI Accession
- NM_001861, NP_001852
Target
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