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Human COX4I1 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)

This is a Cytochrome C Oxidase Subunit IV Isoform 1 plasmid from OriGene with ORF insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: 510 bp. Transient expression. Suitable for PExp. Bacterial selection: Kanamycin. Grade: End-sequenced.
OriGene
Catalog No. ABIN5342524
Supplier Product No.: rc209374
$445.50
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 8 to 11 Business Days

Quick Overview for Human COX4I1 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN5342524)

Gene

COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Fusion tag

Myc-DYKDDDDK Tag

Application

Protein Expression (PExp)

Insert

ORF

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-Entry

Promoter

CMV Promoter

Bacterial Resistance

Kanamycin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    rc209374

    Supplier

    OriGene

    Purpose

    Mammalian Vector with ORF clone of Human cytochrome c oxidase subunit IV isoform 1 (COX4I1)

    Specificity

    Restriction Site: SgfI-MluI

    Grade

    End-sequenced

    Components

    The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

    Insert Length

    510 bp

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    4 °C/-20 °C
  • Target

    COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

    Background

    Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.

    NCBI Accession

    NM_001861, NP_001852
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